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产品目录

MroXI内切酶图片
产品货号:
GS2182
中文名称:
MroXI内切酶
英文名称:
MroXI
产品规格:
200U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesGAANN↑NNTTC
来源Micrococcus roseus X
Assayed onLambda DNA
Optimal bufferW?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)
Optimal temperature37℃
Storage conditions10mMTris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃.
LigationsAfter 5-fold overdigestion with enzyme more than 50%of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃.
Methlation effectMethylation sensitivity not tested
Thermal inactivationenzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible EndsAsp700I,PdmI,XmnI

相关搜索:MroXI内切酶MroXI限制性内切酶
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